D-荧光素钾盐常见问题及解答
D-Luciferin Potassium Salt--FAQ's(Frequently Asked Questions)
Is there a difference between firefly luciferin, beetle luciferin, and D-Luciferin from Cellcyto?
No. D-Luciferin from Cellcyto, firefly luciferin, and beetle luciferin are all names for the same chemical, (S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid. We only provide D-Luciferin Potassium Salt for in vivo and in vitro analysis.
For what applications is Cellcyto D-Luciferin suitable?
Cellcyto D-Luciferin is used in all luciferase applications including gene reporter assays, hygene testing, sanitation monitoring, pyrosequencing, and whole animal imaging.
How is D-Luciferin prepared for whole animal imaging?
D-Luciferin salts are easily soluble up to 30mg/mL in unbuffered water and many preps simply dissolve the luciferin potassium salt at 3-15 mg/mL for i.p. administration.
If a buffering system is to be used, pH and time are very important as D-Luciferin is relatively unstable at low pH (<6.5, hydrolysis) and high pH (>7.5, conversion to L-Luciferin).
What is the difference between the free acid, sodium salt and potassium salt?
The main difference between the free acid and the salt forms is the solubility and dissolution characteristics. D-Luciferin free acid has very low solubility in water and dissolves very slowly into buffered solutions. The potassium salts dissolves quite easily and quickly into water.
How should Cellcyto Technologies D-Luciferin Potassium Salt be stored?
D-Luciferin Potassium Salt should be stored at -20°C, protected from light for two years..
High quality allows the D-Luciferin Potassium Salt to be shipped at room temperature and stored at room temperature for short periods of time.
How stable is D-luciferin in solution?
Frozen (-80°C) samples have been reported to be suitable for use after a year. Liquid samples at room temperature show degradation of up to 0.25% per day with the degradation route being pH dependant. If oxygen is present in the solution, dehydroluciferin will form.
High Quality D-Luciferin at an Affordable Price
Cellcyto provides D-Luciferin for use in research applications ranging from reporter gene assays to whole animal assays. We produce high purity Luciferin to ensure the highest signal, lowest background, highest reproducibility, and lowest sensitivity levels for your application.
D-Luciferin Potassium Salt, minimum 99% by achiral HPLC.
1、D-荧光素适合做哪些实验？
D-荧光素钾盐作为Luciferase的作用底物，常用于以下实验：1)报告基因分析；2)In vivo imaging （动物活体成像）；3)In vitro imaging （细胞体外分析）；4)ATP测定；5)其它luciferase及其基因相关的分析和研究工作
2、D-荧光素应用于活体成像的基本原理
细胞中D-荧光素酶参与的化学发光反应遵守酶促反应的基本原理，在底物D-荧光素钾过量的情况下，反应产生的光子数（发光量）与细胞中的D-荧光素酶的量成正相关，从而可以推算出细胞的数量。
3、D-荧光素钾盐溶液的稳定性
D-荧光素钾盐易溶于水和缓冲液，溶解度可高达25mg/mL。一般使用浓度为3-15 mg/mL。溶液的pH值、溶液中的氧气和保存时间对其保存过程中的稳定性非常重要。当溶液的pH<6.5（发生水解作用）或>7.5（发生消旋化作用，D型转化为L型）的情况下，D-荧光素钾盐相对不稳定。如果溶液中存在少量的氧气，将加速D-荧光素钾的降解速度。
D-荧光素配成溶液后，在最佳的保存条件（-80°C，最佳pH，无氧气）下，存在固定的降解速度：0.2%/天，因此配成溶液保存的D-荧光素应尽量在1年内使用完。
有水存在的情况下，主要发生消旋化作用，即D-荧光素转化成L-荧光素，有报道称L-荧光素对荧光素酶催化的酶促反应有抑制作用。
4、D-荧光素、D-荧光素钠和D-荧光素钾之间的区别
主要区别在于溶解和分散性能。D-荧光素钾盐在水和缓冲液中的溶解度高，且溶解速度较快，故在一般的生物学试验中常用D-荧光素钾盐。
5、D-荧光素钾盐的保存和运输条件
保存条件：≤-20°C，避光，保持干燥。（在不打开包装的条件下，可保存两年时间）。
运输条件：高纯度的D-荧光素钾盐可在短时间内常温条件下保存，运输条件为常温。
原装（没有打开包装）荧光素的包装瓶中充有氩气，保证其稳定性，打开包装后，请严格按照保存条件保存。
D-荧光素钾盐具有易溶于水的特性，其暴露于空气中，易吸潮。在称量过程中，请考虑其易吸潮的特性，先进行温度平衡，再快速称取。
